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      首頁 >> 產品 >> 抗體抗原 >> 標簽和內參 >> HA Antibody
      HA Antibody
      產品編號: ICP0750
      • 100.00μg

      HA Antibody


      Product Description


      This high affinity anti-HA antibody is developed using HA peptide (YPYDVPDYA) conjugated to KLH and affinity-purified with the HA peptide. The antibody is useful for detection, isolation and localization of the HA-tag proteins.

      Western-blot analysis of the HA-tag protein (50 kd) expressed cell lysate and  affinity enriched by IP,  with HA-tag antibody, HRP conjugates (ICP0751) 1: Molecular marker 2: 10 uL of HA-tag protein in lysate (original cell lysate) 3: 10 uL of His-tag protein in lysate (after IP depletion)  4: 10 uL of the HA-tag protein in 100 uL of the eluting buffer enriched with 25 ug of the anti-His tag (ICP0750) from 2 mL of the original lysate.
      Western blot analysis of the cell extract with expression of HA-tag proteins (B-F) and without expression of HA (A).
      500 μg/mL in PBS, 50% glycerol
      GST peptide EEHLYERDEGDKWR KLH conjugates
      Antigen-specific affinity chromatography
      The antibody recognizes HA-tag proteins.
      ELISA (1:2500); WB (1:1000); IP (5 μg/sample)
      Scientific Description
      Hemaglutinin peptide (HA) is frequently used as an affinity tag to facilitate the detection, isolation and localization of the HA-fusion proteins.
      Storage & Stability
      Product is stable for several weeks at 4°C. For extended storage, store product at -20°C.Expiration date is one year from date of shipping if stored properly.
      Product Specific References
      1. Cancer Res. 2010. 70: 10445.doi: 10.1158/0008-5472.CAN-10-3040

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