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      首頁 >> 產品 >> 抗體抗原 >> 標簽和內參 >> His-Tag, Antibody
      His-Tag, Antibody
      產品編號: ICP0850
      • 100.00μg
        ¥1250.00
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      詳細介紹

      His Tag Antibody

      Catalog #

      Product Description

      ICP0850

      This high affinity His-tag antibody is developed using a polyhistidine peptide (HHHHHH) conjugated to KLH and affinity-purified with the HHHHHH peptide on agarose. The antibody is useful for detection, affinity isolation, enrichment  and localization of His-tag proteins.

      Western-blot with His-tag  antibody, HRP (ICP0851) of the His-tag protein (50 kDa) expressed cell lysate and enriched His-tag protein by IP with anti-His-tag (ICP0850)     1: Molecular marker                                         2: 10 μL of His-tag protein in lysate (original cell lysate)                                               3: 10 μL of His-tag protein in lysate (after IP)                                                                           4: 10 μL of the His-tag protein in 100 μL of the IP eluting buffer, enriched with 25 μg of the anti-His tag (ICP0850) from 2 mL of the original lysate
      Species
      Rabbit
      Formulation
      250 μg/mL in PBS, 50% glycerol
      Immunogen
      Polyhistidine peptide (HHHHHH) KLH conjugates
      Purification
      Antigen-specific affinity chromatography
      Specificity
      The antibody recognizes His-tag proteins.
      Applications
      ELISA (0.25-1 μg/mL); IP ( 1-10 μg, based on the sample size) ; WB (0.25-1.0 μg/mL); IHC (5 μg/mL)
      Scientific Description
      Polyhistidine peptide is frequently used as an affinity tag to facilitate the isolation of the 6H-fusion proteins using a metal-chelate matrix.
      Storage & Stability
      Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.


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