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      首頁 >> 產品 >> 測試盒 >> 蛋白激酶活性試劑盒 >> Broad Spectrum Serine/Threonine Kinase Assay Kit
      Broad Spectrum Serine/Threonine Kinase Assay Kit
      產品編號: ICP0248
      • 96 Assays/Kit
        ¥4350.00
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      詳細介紹

      Broad Spectrum Serine/Threonine Kinase Assay Kit

      Catalog#

      Product Description

      ICP0248

      This product is designed for testing the activity of a variety of serine/theonine kinases (STK) (see Table 1) and screening the inhibitors/activators of corespondinging kinases. The assay kit is non-radioactive but colorimetric.

      The principle of the assay:
      The kinase substrates is immobilized onto the 96-well plate. The kinases use ATP to phosphorylate the substrate on the plate. The phosphorylated product is detected by the phospho-substrate specific antibodies. See Figure1 for the principle illustration.
      Formulation
      1x96 assays or 5x96 assays
      Applications
      Assay for purified or partially purified serine/threonine kinases listed in Table 1
      Assay for inhibitors of the serine/threonine kinases listed in Table 1
      Storage & Stability
      Product is stable for 30 days at room remperature. 6 months at 4°C.
      Kit Compoentes
      1. 96-well Substrate Microtiter Plate (Part#: KA101)
      2. Phosphospecific Substrate Antibody(Part#: KA102)
      3. Anti-Rabbit IgG: HRP Conjugate(Part#: KA103)
      4. Antibody Dilution Buffer (Part#:KA104)
      5. Kinase Assay Dilution Buffer (Part#:KA105)
      6. ATP(Part#: KA106)
      7. 20X Wash Buffer (Part#:KA107)
      8. TMB Substrate (Part#:KA108)
      9. Stop Solution (Part#:KA109)

      Figure 1   Assay Principle

      Table 1 Activity of STKs Tested by the Assay Kit


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