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      首頁 >> 產品 >> 標記抗體抗原 >> Agarose偶聯填料 >> Papain Agarose
      Papain Agarose
      產品編號: ICP1948
      • 10ml

      Papain Agarose

      Catalog#            ICP1948
      Product Description

      Papain is conjugated to agarose bead via amine linkage. The product can be repetitively used as an immobilized papain for various proteolysis applications. It can efficiently hydrolyze antibodies into Fc and Fab fragments.




       Sample Data


      Figure1 Preparation of Goat anti-Mouse IgG Fab


      One gram of purified Goat anti-Mouse IgG (lane 1) was loaded onto the Papain Agarose column (10 mL) at a speed of 0.5 mL/min. The flow-through (lane 2) was subsequently affinity bound to a Mouse IgG Agarose column, and the pure Fab fragment (lane 3) was then purified. 

      Papain Immobilized

      60-300 units/mL of papain (30 mg/mL) is covalently linked to Sepharose CL-4B


      5 mL of wet agarose beads suspended with 5 mL of glycerol



      Enzymatic hydrolysis of proteins

      Preparation of antibody Fc/Fab fragments


      Storage & Stability

      Product is stable for 4 weeks at ambient temperature. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if properly stored.




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